nanoCLAMP slyD-A1 is a single domain antibody mimetic recognizing E. coli SlyD. SlyD is a histidine-rich FKBP-type peptidyl-prolyl cis-trans isomerase and is often the predominant contaminant of 6xHis-tagged proteins eluted from Ni-NTA resin. nanoCLAMP slyD-A1 anti-NusA beads are useful for SlyD removal, SlyD depletion, SlyD isolation, SlyD purification, SlyD chromatography, and SlyD immunoprecipitation. Removal of SlyD contamination by nanoCLAMP slyD-A1(Resin) is an alternative to purification of 6xHis-tagged proteins with cobalt resins or expression of 6xHis-tagged proteins in specialized E. coli strains such as LOBSTR, NiCo21(DE3), and NiCo22(DE3).
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Affinity chromatography resin for E. coli SlyD. nanoCLAMP slyD-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of ca. 3.6 mg (171 nmol) SlyD per ml resin. 0.75 ml resin in 1.5 ml suspension.