nanoCLAMP slyD-A1


nanoCLAMP slyD-A1 is a single domain antibody mimetic recognizing E. coli SlyD. SlyD is a histidine-rich FKBP-type peptidyl-prolyl cis-trans isomerase and is often the predominant contaminant of 6xHis-tagged proteins eluted from Ni-NTA resin. nanoCLAMP slyD-A1 anti-NusA beads are useful for SlyD removal, SlyD depletion, SlyD isolation, SlyD purification, SlyD chromatography, and SlyD immunoprecipitation. Removal of SlyD contamination by nanoCLAMP slyD-A1(Resin) is an alternative to purification of 6xHis-tagged proteins with cobalt resins or expression of 6xHis-tagged proteins in specialized E. coli strains such as LOBSTR, NiCo21(DE3), and NiCo22(DE3). 

Products 

Catalog No. Description Academic Price Commercial Price (including license fee) Quantity
nC-slyD-A1(Resin)

Affinity chromatography resin for E. coli SlyD. nanoCLAMP slyD-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of ca. 3.6 mg (171 nmol) SlyD per ml resin. 0.75 ml resin in 1.5 ml suspension.

 

$475 $975

Applications 

Targets
Consensus Target
Confirmed Targets
Non-Reactive Targets
Biophysical Data
Extinction Coefficients
Variantε (280 nm)SolventDetermination Method
36,130 M-1cm-1
6 M Guanidine HCl
36,440 M-1cm-1
Water

Variants 

nanoCLAMP slyD-A1(Cys) nanoCLAMP slyD-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys
Conjugates
nanoCLAMP slyD-A1(Resin) slyD-A1(Cys) coupled to Sulfolink™ resin