Nectagen

nanoCLAMP nusA-A1

nusA-A1 is a single domain antibody mimetic recognizing NusA, NusA fusions, and NusA-tagged proteins. NusA is a transcription termination and antitermination protein from E. coli and is often used as a solubility enhancing tag for heterologously expressed proteins. nanoCLAMP nusA-A1 anti-NusA beads are useful for NusA purification, NusA isolation, NusA chromatography, NusA immunoprecipitation, NusA depletion, and NusA removal. nanoCLAMP nusA-A1 is an alternative to anti-NusA antibodies.  

Products 

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-nusA-A1(Cys)	nanoCLAMP nusA-A1 recognizing NusA. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-nusA-A1(Resin)	Affinity chromatography resin for NusA. nanoCLAMP nusA-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 2.9 mg (40 nmol) SUMO-NusA fusion per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for NusA purification, NusA immunoprecipitation (IP), NusA chromatography, NusA isolation, NusA pulldown, NusA removal, and NusA depletion. Recognizes NusA, NusA-fusions and NusA-tagged proteins.  Alternative to NusA antibody beads.	$475	$975	Quantity
pET(nC-nusA-A1)	T7 expression plasmid for nanoCLAMP nusA-A1. 200 ng lyophilized DNA.	$65	$2000	Quantity

Applications 

Figure	Application	Outcome
	NusA purification by affinity chromatography with nanoCLAMP nusA-A1(Resin), batch mode from E. coli lysate	Successful
	Determination of dissociation constant of nusA-A1(Cys) for NusA by microscale thermophoresis	Successful
	NusA sandwich ELISA with immobilized nusA-A1(Cys)	Successful
	Western blot analysis of recombinant NusA using nusA-A1(Cys) as primary and anti-His-HRP as secondary	Successful

Variants 

nanoCLAMP nusA-A1(Cys)

nanoCLAMP nusA-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys