Nectagen

nanoCLAMP mCher-A2

nanoCLAMP mCher-A2 is a single domain antibody mimetic recognizing mCherry, mCherry fusions and mCherry-tagged proteins. Validated applications include affinity chromatography to purify mCherry protein and sandwich ELISA to detect mCherry and mCherry fusions. nanoCLAMPmCher-A1 anti-mCherry beads are useful for mCherry purification, mCherry immunoprecipitation (IP), mCherry pulldown, mCherry chromatography and mCherry isolation. Like mCherry antibodies and mCherry nanobodies, nanoCLAMP mCher-A2 binds mCherry tightly and selectively but, unlike these alternatives, nanoCLAMP mCher-A2 releases mCherry under mild conditions to support the purification of functional mCherry and mCherry-tagged protein complexes. 

Products 

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-mCher-A2(Cys)	nanoCLAMP mCher-A2 recognizing mCherry. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-mCher-A2(Resin)	Affinity chromatography resin for mCherry. nanoCLAMP mCher-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 2.2 mg (75 nmol) mCherry per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for mCherry purification, mCherry immunoprecipitation (IP), mCherry chromatography, mCherry isolation and mCherry pulldown. Recognizes mCherry, mCherry-fusions and mCherry-tagged proteins.  Alternative to mCherry antibody beads and mCherry nanobody beads.	$475	$975	Quantity

Applications 

Figure	Application	Outcome
	mCherry purification by affinity chromatography with nanoCLAMP mCher-A2(Resin), batch mode from E. coli lysate	Successful

Variants 

nanoCLAMP mCher-A2(Cys)

nanoCLAMP mCher-A2 with N-terminal His-tag and C-terminal GS13 linker and unique Cys