Nectagen

nanoCLAMP malE-A1

nanoCLAMP malE-A1 is a single domain antibody mimetic recognizing maltose binding protein (MBP). Validated applications include affinity chromatography and sandwich ELISA. nanoCLAMP malE-A1 anti-MBP beads are useful for MBP purification, MBP immunoprecipitation (IP), MBP pulldown, MBP chromatography, MBP isolation, MBP removal and MBP depletion. nanoCLAMP malE-A1(Resin) is an alternative to amylose resin or amylose beads and represents an orthogonal purification method as a polishing step for purified MBP fusion proteins. 

Products 

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-malE-A1(Cys)	nanoCLAMP malE-A1 recognizing MBP. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-malE-A1(Resin)	Affinity chromatography resin for maltose binding protein (MBP). nanoCLAMP malE-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 0.9 mg (20 nmol) MBP per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for MBP purification, MBP immunoprecipitation (IP), MBP chromatography, MBP isolation, MBP pulldown, MBP removal, and MBP depletion. Recognizes MBP, MBP-fusions and MBP-tagged proteins.  Alternative to MBP antibody beads or amylose beads.	$475	$975	Quantity
pET(nC-malE-A1)	T7 expression plasmid for nanoCLAMP malE-A1. 200 ng lyophilized DNA.	$65	$2000	Quantity

Applications 

Figure	Application	Outcome
	MBP purification by affinity chromatography with nanoCLAMP malE-A1(Resin), batch mode from E. coli lysate	Successful
	Maltose binding protein (MBP) sandwich ELISA with immobilized malE-A1(Cys)	Successful
	Determination of binding constant of malE-A1(Cys) for MBP by microscale thermophoresis	Successful

Variants 

nanoCLAMP malE-A1(Cys)

nanoCLAMP malE-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys