nanoCLAMP lacZ-A2


nanoCLAMP lacZ-A2 is a single domain antibody recognizing beta-galactosidase (lacZ) and lacZ fusion proteins. nanoCLAMP lacZ-A2 anti-lacZ beads are useful for lacZ purification, lacZ immunoprecipitation (IP), and lacZ isolation. 

Products 

Catalog No. Description Academic Price Commercial Price (including license fee) Quantity
nC-lacZ-A2(Cys)

nanoCLAMP lacZ-A2 recognizing beta-galactosidase. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).

 $275 $525
nC-lacZ-A2(Resin)

Affinity chromatography resin for beta-galactosidase (lacZ). nanoCLAMP lacZ-A2 coupled to 6% agarose beads via thioether bond to C-terminal cysteine.  Binding capacity of > 4.1 mg (8 nmol) beta-galactosidase per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for lacZ purification, lacZ immunoprecipitation (IP), lacZ chromatography, lacZ isolation, lacZ pulldown, lacZ removal, and lacZ depletion. Recognizes lacZ, lacZ-fusions and lacZ-tagged proteins.  Alternative to lacZ antibody beads.

 $475 $975

Applications 

Figure Application Outcome
SDS PAGE of lacZ purification with nanoCLAMP lacZ-A1 affinity chromatography Beta-galactosidase purification by affinity chromatography with nanoCLAMP lacZ-A2(Resin), batch mode from E. coli lysate Successful
Targets
Consensus Target
β-galactosidase
Confirmed Targets
β-galactosidase
Non-Reactive Targets
Biophysical Data
Extinction Coefficients
Variantε (280 nm)SolventDetermination Method
nanoCLAMP lacZ-A2(Cys)
29,160 M-1cm-1
6 M Guanidine HCl

Pace et al. 1995
nanoCLAMP lacZ-A2(Cys)
29,450 M-1cm-1
Water

Pace et al. 1995

Variants 

nanoCLAMP lacZ-A2(Cys) Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).
Conjugates
nanoCLAMP lacZ-A2(Resin) lacZ-A2(Cys) coupled to Sulfolink™ resin
Reference(s)
Reference Title PMID/ISBN
Suderman et al. 2017 Development of Polyol-Responsive Antibody Mimetics for Single-Step Protein Purification 28428153