nanoCLAMP GFP-A1

nanoCLAMP GFP-A1 is a single domain antibody mimetic recognizing GFP, GFP fusions and GFP-tagged proteins. nanoCLAMP GFP-A1 recognizes GFP, eGFP, Emerald GFP, GFPMut2, and BFP but does not recognize YFP, CFP, mCherry or dsRed. Validated applications include affinity chromatography to purify GFP protein and sandwich ELISA to detect GFP and GFP fusions. nanoCLAMP GFP-A1 anti-GFP beads are useful for GFP purification, GFP immunoprecipitation (IP), GFP pulldown, GFP chromatography and GFP isolation. Like GFP antibodies, GFP nanobodies, and GFP traps, nanoCLAMP GFP-A1 binds GFP tightly and selectively but, unlike these alternatives, nanoCLAMP GFP-A1 releases target protein under gentle conditions in support the purification of functional GFP and GFP-tagged protein complexes.

Products

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-GFP-A1(Cys)	nanoCLAMP GFP-A1 recognizing GFP, eGFP, GFPmut2, and BFP. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-GFP-A1(Resin)	Affinity chromatography resin for GFP, eGFP, GFPmut2, and BFP. nanoCLAMP GFP-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 2.2 mg (75 nmol) GFP per ml resin. 0.75 ml of resin in 1.5 ml suspension. Useful for GFP purification, GFP immunoprecipitation (IP), GFP chromatography, GFP isolation and GFP pulldown. Recognizes GFP, GFP-fusions and GFP-tagged proteins. Alternative to GFP antibody beads, GFP nanobody beads, and GFP trap beads.	$475	$975	Quantity
pET(nC-GFP-A1)	T7 expression plasmid for nanoCLAMP GFP-A1. 200 ng lyophilized DNA.	$65	$2000	Quantity

Applications

Figure	Application	Outcome
	GFP purification by affinity chromatography with nanoCLAMP GFP-A1(Resin), batch mode from E. coli lysate	Successful
	Determination of cross-reactivity of GFP-A1(Resin) for various fluorescent proteins	Successful
	GFP sandwich ELISA with immobilized GFP-A1(Cys)	Successful
	Determination of dissociation constant of GFP-A1(Cys) for GFP by microscale thermophoresis	Successful

Targets
Consensus Target	GFP
Confirmed Targets	GFP, EGFP, GFPMut2, BFP, Emerald GFP
Non-Reactive Targets	YFP, mCherry, DsRed, CFP

Biophysical Data

Binding Data

Type	Value	Target	App
Kd	32 nM	GFP	Determination of dissociation constant of GFP-A1(Cys) for GFP by microscale thermophoresis

Extinction Coefficients

Variant	ε (280 nm)	Solvent	Determination Method
nanoCLAMP GFP-A1(Cys)	41,820 M^-1cm^-1	6 M Guanidine HCl	Pace et al. 1995
nanoCLAMP GFP-A1(Cys)	41,940 M^-1cm^-1	Water	Pace et al. 1995

Variants

nanoCLAMP GFP-A1(Cys)

nanoCLAMP GFP-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys

Conjugates

nanoCLAMP GFP-A1(Resin)

GFP-A1(Cys) coupled to Sulfolink™ resin

Fusions

nanoCLAMP GFP-A1/mCherry

GFP-A1 fused to mCherry

Reference(s)

Reference	Title	PMID/ISBN
Suderman et al. 2017	Development of Polyol-Responsive Antibody Mimetics for Single-Step Protein Purification	28428153