nanoCLAMP AVD-A1

nanoCLAMP AVD-A1 is a single domain antibody mimetic recognizing Avidin and Neutravidin. Validated applications include affinity chromatography and sandwich ELISA. nanoCLAMP AVD-A1 anti-Avidin beads are useful for Avidin purification, Avidin immunoprecipitation (IP), Avidin pulldown, Avidin chromatography and Avidin isolation. nanoCLAMP AVD-A1(Resin) is an alternative to biotin and iminobiotin beads but allows elution under milder conditions.

Products

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-AVD-A1(Cys)	nanoCLAMP AVD-A1 recognizing avidin and neutravidin. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-AVD-A1(Resin)	Affinity chromatography resin for avidin and neutravidin. nanoCLAMP AVD-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 2.6 mg (40 nmol) avidin per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for avidin purification, avidin immunoprecipitation (IP), avidin chromatography, avidin isolation, avidin pulldown, avidin removal, and avidin depletion. Recognizes avidin, avidin-fusions and avidin-tagged proteins. Alternative to avidin antibody beads, biotin beads, and iminobiotin beads.	$475	$975	Quantity
pET(nC-AVD-A1)	T7 expression plasmid for nanoCLAMP AVD-A1. 200 ng lyophilized DNA.	$65	$2000	Quantity

Applications

Figure	Application	Outcome
	Avidin purification by affinity chromatography with nanoCLAMP AVD-A1(Resin), batch mode with E. coli lysate	Successful
	Purification of neutravidin from E. coli lysate by batch affinity chromatography with AVD-A1(Resin)	Successful
	Avidin sandwich ELISA with immobilized AVD-A1(Cys)	Successful
	Determination of dissociation constant of AVD-A1(Cys) for Avidin by microscale thermophoresis	Successful

Targets
Consensus Target	Avidin
Confirmed Targets	Avidin, Neutravidin
Non-Reactive Targets

Biophysical Data

Binding Data

Type	Value	Target	App
Kd	20 nM	Avidin	Determination of dissociation constant of AVD-A1(Cys) for Avidin by microscale thermophoresis

Extinction Coefficients

Variant	ε (280 nm)	Solvent	Determination Method
nanoCLAMP AVD-A1(Cys)	43,100 M^-1cm^-1	6 M Guanidine HCl	Pace et al. 1995
nanoCLAMP AVD-A1(Cys)	43,430 M^-1cm^-1	Water	Pace et al. 1995

Variants

nanoCLAMP AVD-A1(Cys)

nanoCLAMP AVD-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys

Conjugates

nanoCLAMP AVD-A1(Resin)

AVD-A1(Cys) coupled to Sulfolink™ resin

Reference(s)

Reference	Title	PMID/ISBN
Suderman et al. 2017	Development of Polyol-Responsive Antibody Mimetics for Single-Step Protein Purification	28428153