nanoCLAMP GFP-A1




nanoCLAMP GFP-A1 is a single domain antibody mimetic recognizing GFP, GFP fusions and GFP-tagged proteins. nanoCLAMP GFP-A1 recognizes GFP, eGFP, Emerald GFP, GFPMut2, and BFP but does not recognize YFP, CFP, mCherry or dsRed. Validated applications include affinity chromatography to purify GFP protein and sandwich ELISA to detect GFP and GFP fusions. nanoCLAMP GFP-A1 anti-GFP beads are useful for GFP purification, GFP immunoprecipitation (IP), GFP pulldown, GFP chromatography and GFP isolation. Like GFP antibodies, GFP nanobodies, and GFP traps, nanoCLAMP GFP-A1 binds GFP tightly and selectively but, unlike these alternatives, nanoCLAMP GFP-A1 releases target protein under gentle conditions in support the purification of functional GFP and GFP-tagged protein complexes.  

Products 

Catalog No. Description Academic Price Commercial Price Quantity
nC-GFP-A1(Cys)

nanoCLAMP GFP-A1 recognizing GFP, eGFP, GFPmut2, and BFP. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).

$275 $525
nC-GFP-A1(Resin)

Affinity chromatography resin for GFP, eGFP, GFPmut2, and BFP. nanoCLAMP GFP-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 2.2 mg (75 nmol) GFP per ml resin. 0.75 ml of resin in 1.5 ml suspension. Useful for GFP purification, GFP immunoprecipitation (IP), GFP chromatography, GFP isolation and GFP pulldown. Recognizes GFP, GFP-fusions and GFP-tagged proteins.  Alternative to GFP antibody beads, GFP nanobody beads, and GFP trap beads.

$475 $975
pET(nC-GFP-A1)

T7 expression plasmid for nanoCLAMP GFP-A1. 200 ng lyophilized DNA.

$65 $2000

Applications 

Targets
Consensus Target
Confirmed Targets
Non-Reactive Targets

Variants 

nanoCLAMP GFP-A1(Cys) nanoCLAMP GFP-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys
Conjugates
nanoCLAMP GFP-A1(Resin) GFP-A1(Cys) coupled to Sulfolink™ resin
Fusions
nanoCLAMP GFP-A1/mCherry GFP-A1 fused to mCherry
References
Reference Title PMID/ISBN
Suderman et al. 2017 Development of Polyol-Responsive Antibody Mimetics for Single-Step Protein Purification 28428153