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Figure 1

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NusA affinity reagent

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Analysis of the interaction between nusA-A1(Cys) and NusA-GFP by microscale thermophoresis. Binding of nusA-A1(Cys) to NusA-GFP was quantified in PBS-T (0.05% Tween). NusA-GFP was held at 100 nM while the concentration of nusA-A1(Cys) was varied from 10 uM to 0.305 nM while the migration of the fluorescent protein was measured upon local heating using a Monolith NT.115 Pico with 80% Laser Power, 20% LED power, at 25C.