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Figure 1



Analysis of the interaction between GFP-A1 and GFP by microscale thermophoresis. Binding of GFP-A1 to GFP was quantified in PBS-T (0.05% Tween). GFP was held at 10 nM while the concentration of GFP-A1 was varied from 5 µM to 0.153 nM while the migration of the fluorescent protein was measured upon local heating using a Monolith NT.115 Pico with 20% Laser Power, 15% LED power, at 25C.