Western blot using nanoCLAMP and anti-His-HRP |
2 |
Sandwich ELISA |
5 |
Preparation of E. coli lysate with B-PER solution |
0 |
Ni-NTA purification of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets |
0 |
Ni-NTA purification and on-column renaturation of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets |
0 |
Induction of protein expression from E. coli NEc1 - frozen cell bank to cell pellet |
0 |
Determination of cross-reactivity of nanoCLAMPs for similar proteins by immunoprecipitation |
1 |
Contaminant depletion using nanoCLAMP(Resin) |
2 |
Conjugation of nanoCLAMP(Cys) to a maleimide label |
0 |
Conjugation of a nanoCLAMP to SulfoLink™ resin |
0 |
Calculation of protein molar extinction coefficient (ɛ) with values from Pace et al. 1995 |
0 |
Affinity purification from E.coli lysates using nanoCLAMP(Resin) |
10 |