|
Western blot using nanoCLAMP and anti-His-HRP |
2 |
|
Sandwich ELISA |
5 |
|
Preparation of E. coli lysate with B-PER solution |
0 |
|
Ni-NTA purification of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets |
0 |
|
Ni-NTA purification and on-column renaturation of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets |
0 |
|
Induction of protein expression from E. coli NEc1 - frozen cell bank to cell pellet |
0 |
|
Determination of cross-reactivity of nanoCLAMPs for similar proteins by immunoprecipitation |
1 |
|
Contaminant depletion using nanoCLAMP(Resin) |
2 |
|
Conjugation of nanoCLAMP(Cys) to a maleimide label |
0 |
|
Conjugation of a nanoCLAMP to SulfoLink™ resin |
0 |
|
Calculation of protein molar extinction coefficient (ɛ) with values from Pace et al. 1995 |
0 |
|
Affinity purification from E.coli lysates using nanoCLAMP(Resin) |
10 |
