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Home » Applications Data »

Protocols


Protocols describe methods for using Nectagen products. Protocols are contributed by Nectagen employees or individual users. Users are eligible to earn Nectagen points after the review and acceptance of submissions.  Learn more about Nectagen points 

Protocol Apps Using Protocol
Western blot using nanoCLAMP and anti-His-HRP 2
Sandwich ELISA 5
Preparation of E. coli lysate with B-PER solution 0
Ni-NTA purification of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets 0
Ni-NTA purification and on-column renaturation of denatured His-tagged nanoCLAMP proteins from NEc1 cell pellets 0
Induction of protein expression from E. coli NEc1 - frozen cell bank to cell pellet 0
Determination of cross-reactivity of nanoCLAMPs for similar proteins by immunoprecipitation 1
Contaminant depletion using nanoCLAMP(Resin) 2
Conjugation of nanoCLAMP(Cys) to a maleimide label 0
Conjugation of a nanoCLAMP to SulfoLink™ resin 0
Calculation of protein molar extinction coefficient (ɛ) with values from Pace et al. 1995 0
Affinity purification from E.coli lysates using nanoCLAMP(Resin) 10