Neutravidin |
Affinity Chromatography |
Successful |
Purification of neutravidin from E. coli lysate by batch affinity chromatography with AVD-A1(Resin) |
Avidin |
Biophysical Constant Measurement |
Successful |
Determination of dissociation constant of AVD-A1(Cys) for Avidin by microscale thermophoresis |
Avidin |
ELISA |
Successful |
Avidin sandwich ELISA with immobilized AVD-A1(Cys) |
GFP |
Affinity Chromatography |
Successful |
GFP purification by affinity chromatography with nanoCLAMP GFP-A1(Resin), batch mode from E. coli lysate |
GFP |
Biophysical Constant Measurement |
Successful |
Determination of dissociation constant of GFP-A1(Cys) for GFP by microscale thermophoresis |
GFP |
ELISA |
Successful |
GFP sandwich ELISA with immobilized GFP-A1(Cys) |
MBP |
Affinity Chromatography |
Successful |
MBP purification by affinity chromatography with nanoCLAMP malE-A1(Resin), batch mode from E. coli lysate |
MBP |
Biophysical Constant Measurement |
Successful |
Determination of binding constant of malE-A1(Cys) for MBP by microscale thermophoresis |
MBP |
ELISA |
Successful |
Maltose binding protein (MBP) sandwich ELISA with immobilized malE-A1(Cys) |
NusA |
Affinity Chromatography |
Successful |
NusA purification by affinity chromatography with nanoCLAMP nusA-A1(Resin), batch mode from E. coli lysate |
NusA |
Biophysical Constant Measurement |
Successful |
Determination of dissociation constant of nusA-A1(Cys) for NusA by microscale thermophoresis |
NusA |
ELISA |
Successful |
NusA sandwich ELISA with immobilized nusA-A1(Cys) |
SUMO (yeast) |
Affinity Chromatography |
Successful |
SUMO purification by affinity chromatography with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate |
SUMO (yeast) |
Biophysical Constant Measurement |
Successful |
Determination of dissociation constant of SMT3-A1(Cys) for SMT3 (yeast SUMO) by microscale thermophoresis |
SUMO (yeast) |
ELISA |
Successful |
SMT3 (yeast SUMO) sandwich ELISA with immobilized SMT3-A1(Cys) |
Avidin |
Affinity Chromatography |
Successful |
Avidin purification by affinity chromatography with nanoCLAMP AVD-A1(Resin), batch mode with E. coli lysate |
GFP |
Immunoprecipitation |
Successful |
Determination of cross-reactivity of GFP-A1(Resin) for various fluorescent proteins |
SUMO (yeast) |
Western |
Successful |
Western blot analysis of recombinant SMT3 (yeast SUMO) using SMT3-A1(Cys) as primary and anti-His-HRP as secondary |
NusA |
Western |
Successful |
Western blot analysis of recombinant NusA using nusA-A1(Cys) as primary and anti-His-HRP as secondary |
β-galactosidase |
Affinity Chromatography |
Successful |
Beta-galactosidase purification by affinity chromatography with nanoCLAMP lacZ-A2(Resin), batch mode from E. coli lysate |
mCherry |
Affinity Chromatography |
Successful |
mCherry purification by affinity chromatography with nanoCLAMP mCher-A2(Resin), batch mode from E. coli lysate |
Thioredoxin-1 (E. coli) |
Affinity Chromatography |
Successful |
Thioredoxin purification by affinity chromatography with nanoCLAMP trxA-A1(Resin), batch mode from E. coli lysate |
SlyD |
Affinity Chromatography |
Successful |
SlyD removal from Ni-NTA purified protein with nanoCLAMP slyD-A1(Resin) |
SUMOstar |
Affinity Chromatography |
Successful |
SUMOstar affinity purification with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate |
SlyD |
Affinity Chromatography |
Successful |
Direct removal of SlyD contaminant from IMAC eluates using SlyD-A1(Resin) |