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Abstract | This application involves the identification of antigens in solution by sandwich ELISA using nanoCLAMPs covalently conjugated to maleimide coated microtiter wells via their lone C-terminal Cys. Antigen bound by the immobilized nanoCLAMP is identified by an enzyme-conjugated secondary antibody followed by development with the appropriate secondary substrate. |
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Type |
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Target |
Outcome | Successful |
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Signal | Acceptable |
Cross Reactivity | Acceptable |
Background | Acceptable |
Dynamic Range | Acceptable |
Figure | ImageLegendAnalysis of the interaction between GFP-A1(Cys) and GFP by sandwich ELISA. GFP-A1(Cys) was conjugated to maleimide coated microtiter wells of 96 well strips via the lone C-terminal Cys, at 5 µg/ml. Dilutions of GFP-V5 were incubated on the strips, washed, and then incubated with anti-V5-HRP and developed with TMB Ultra. Reactions were stopped with H2S04 and absorbance measured at A450. The blank wells were processed the same, except they included no GFP-A1(Cys). |
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