Direct removal of SlyD contaminant from IMAC eluates using SlyD-A1(Resin)
Protein eluted from Ni-NTA SF resin was directly applied to SlyD-A1(Resin) without buffer exchange and depleted of SlyD contaminant.
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Removal of SlyD contaminant from Ni-NTA eluate. Protein containing SlyD contaminant was eluted from Ni-NTA SF resin in Qiagen elution buffer, containing 250 mM imidazole (IMAC eluate). Approximately 0.26 mg of protein was applied to 37 µl packed SlyD-A1(Resin) and incubated for 1 h, 4C, rotating, and the flow through (SlyD-A1(Resin) FT) collected and analyzed on 12% SDS-PAGE in reducing sample buffer.