Direct removal of SlyD contaminant from IMAC eluates using SlyD-A1(Resin)


Overview
Abstract
Protein eluted from Ni-NTA SF resin was directly applied to SlyD-A1(Resin) without buffer exchange and depleted of SlyD contaminant.
Type
  • Affinity Chromatography
Specimen
E. coli BL21(DE3)
Target
SlyD
Scores
Outcome
Successful
Signal
Acceptable
Cross Reactivity
Acceptable
Background
Acceptable
Dynamic Range
Acceptable
Results
Figure

Image 

SlyD removal by affinity chromatography

Legend 

Removal of SlyD contaminant from Ni-NTA eluate. Protein containing SlyD contaminant was eluted from Ni-NTA SF resin in Qiagen elution buffer, containing 250 mM imidazole (IMAC eluate). Approximately 0.26 mg of protein was applied to 37 µl packed SlyD-A1(Resin) and incubated for 1 h, 4C, rotating, and the flow through (SlyD-A1(Resin) FT) collected and analyzed on 12% SDS-PAGE in reducing sample buffer.
Methods
Protocol
Contaminant depletion using nanoCLAMP(Resin)
Product Lot(s) Used
Lot Function Academic Price Commercial Price Order Equivalent Product
nC-slyD-A1(Resin)750ul-01 $475 $975