Applications

Applications describe results obtained with Nectagen technology. Nectagen encourages users to submit applications, whether the results are positive or negative, so that other users may make informed decisions about how to use a specific reagent. If a submission is accepted, the author receives points redeemable for discounts.

All applications are contained in a structured database actively maintained by Nectagen to enable efficient searches and comparisons.

Target Type Outcome Title
Avidin Affinity Chromatography Successful Avidin purification by affinity chromatography with nanoCLAMP AVD-A1(Resin), batch mode with E. coli lysate
Avidin Biophysical Constant Measurement Successful Determination of dissociation constant of AVD-A1(Cys) for Avidin by microscale thermophoresis
Avidin ELISA Successful Avidin sandwich ELISA with immobilized AVD-A1(Cys)
Beta-galactosidase Affinity Chromatography Successful Beta-galactosidase purification by affinity chromatography with nanoCLAMP lacZ-A2(Resin), batch mode from E. coli lysate
GFP ELISA Successful GFP sandwich ELISA with immobilized GFP-A1(Cys)
GFP Immunoprecipitation Successful Determination of cross-reactivity of GFP-A1(Resin) for various fluorescent proteins
GFP Affinity Chromatography Successful GFP purification by affinity chromatography with nanoCLAMP GFP-A1(Resin), batch mode from E. coli lysate
GFP Biophysical Constant Measurement Successful Determination of dissociation constant of GFP-A1(Cys) for GFP by microscale thermophoresis
MBP Affinity Chromatography Successful MBP purification by affinity chromatography with nanoCLAMP malE-A1(Resin), batch mode from E. coli lysate
MBP Biophysical Constant Measurement Successful Determination of binding constant of malE-A1(Cys) for MBP by microscale thermophoresis
MBP ELISA Successful Maltose binding protein (MBP) sandwich ELISA with immobilized malE-A1(Cys)
mCherry Affinity Chromatography Successful mCherry purification by affinity chromatography with nanoCLAMP mCher-A2(Resin), batch mode from E. coli lysate
Neutravidin Affinity Chromatography Successful Purification of neutravidin from E. coli lysate by batch affinity chromatography with AVD-A1(Resin)
NusA Affinity Chromatography Successful NusA purification by affinity chromatography with nanoCLAMP nusA-A1(Resin), batch mode from E. coli lysate
NusA Biophysical Constant Measurement Successful Determination of dissociation constant of nusA-A1(Cys) for NusA by microscale thermophoresis
NusA ELISA Successful NusA sandwich ELISA with immobilized nusA-A1(Cys)
NusA Western Successful Western blot analysis of recombinant NusA using nusA-A1(Cys) as primary and anti-His-HRP as secondary
SlyD Affinity Chromatography Successful SlyD removal from Ni-NTA purified protein with nanoCLAMP slyD-A1(Resin)
SUMO (yeast) Affinity Chromatography Successful SUMO purification by affinity chromatography with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate
SUMO (yeast) Biophysical Constant Measurement Successful Determination of dissociation constant of SMT3-A1(Cys) for SMT3 (yeast SUMO) by microscale thermophoresis
SUMO (yeast) ELISA Successful SMT3 (yeast SUMO) sandwich ELISA with immobilized SMT3-A1(Cys)
SUMO (yeast) Western Successful Western blot analysis of recombinant SMT3 (yeast SUMO) using SMT3-A1(Cys) as primary and anti-His-HRP as secondary
SUMOstar Affinity Chromatography Successful SUMOstar affinity purification with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate
Thioredoxin-1 (E. coli) Affinity Chromatography Successful Thioredoxin purification by affinity chromatography with nanoCLAMP trxA-A1(Resin), batch mode from E. coli lysate